Helicobacter. pylori infection is a global health problem that leads to gastriculcers and cancer. The current study aimed to identify some of the factors affecting infection with H. pylori and to study its sensitivity to some antibiotics. This study was conducted in the Endoscopy Unit in the Gastrointestinal Center of Baghdad Teaching Hospital for the period from 25/1/2021 to 4/3/2022.55 isolates of H.pylori were detected. Factors affecting infection with H. pylori that were studied included age, as it reached the highest rate of infection in the age group (11-20years(, with a rate of 33%, as well as gender had an effect on infection, as females showed a higher infection rate than male, it reached 71% and 29%, respectively. Smoking was also associated with infection with H. pylori, by 24%, and the type of food was also associated with infection, by 36%. There was also a relationship between the infection and the place of residence, as the infection appeared in villages higher than in cities, by 58% and 42%,respectively, some immune diseases associated with H. pylori infection were also studied, as it was found that there is a relationship between H. pylori infection and diabetes, with a rate of 41%.The sensitivity test using the Kirby-Bauer disk diffusion method showed that most of the H .pylori isolates are sensitive to the antibiotics under study and gave the highest sensitivity to Rifabutin(99%) and lowest sensitivity to Penicillin(8%).Therefore, early detection and knowledge of the factors affecting the increase in infection with this bacterium, as well as regular antibiotic sensitivity test, helps to limit its spread and improve control of the outbreak of the disease.